Oxy-peptide nucleic acid with a pyrrolidine ring that is configurationally optimized for hybridization with DNA.
نویسندگان
چکیده
Four stereoisomers of oxy-peptide nucleic acids containing ether linkages in the main chain and conformationally-restricted pyrrolidine rings (pyrrolidine-based oxy-PNA = POPNA) were newly synthesized and investigated for binding to DNA. cis-l-POPNA with 9 adenine bases formed the most stable hybrid with dT(9). The POPNA showed high sequence specificity similar to that of the Nielsen-type PNA and sharper melting behavior in hybridization with DNA than the Nielsen-type PNA.
منابع مشابه
Thermodynamic analysis of hybridization between POPNA and DNA.
We have developed a new series of oxy-peptide nucleic acids with pyrrolidine rings of four different types of structural isomers (cis L, cis D, trans L, trans D configurations; POPNAs). Melting curves of adenine nanomers of POPNAs in the presence of the complementary DNA showed the highest Tm value for cis L-POPNA, but in the case of RNA, the highest Tm value was observed for trans L-POPNA. The...
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ورودعنوان ژورنال:
- Chemical communications
دوره 10 شماره
صفحات -
تاریخ انتشار 2004